• Biology: Transcription

  • Sep 18 2024
  • Length: 36 mins
  • Podcast

Biology: Transcription

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  • Summary

  • How is DNA converted into RNA?

    In this episode, we continue our conversation in molecular biology by focusing on the first stage of the Central Dogma: transcription. We explore this process in tremendous detail⎯from the unique proteins involved in initiation, elongation, and termination to the many ways transcription differs between prokaryotes and eukaryotes.


    Part 1: Overview

    - Transcription

    - RNA vs. DNA

    - Prokaryotic and Eukaryotic RNA Polymerases


    Part 2: Initiation

    - The Prokaryotic Promoter: the -35 Sequence and the Pribnow Box

    - The Eukaryotic Promoter: the TATA Box

    - Open and Closed Complexes

    - The Preinitiation Complex

    - Transcription Factors


    Part 2: Elongation

    - The Transcription Bubble

    - The Template and Non-Template Strand


    Part 3: Termination

    - Prokaryotic Termination: Rho-Dependent and Rho-Independent

    - Eukaryotic Termination and the Polyadenylation Signal Sequence

    - Processivity


    Part 4: Eukaryotic Post-Transcriptional Modification

    - Introns, Exons, and Splicing

    - Alternative Splicing

    - The 5' Cap

    - The Poly(A)-Tail
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